Re: p16 immuno staining
Could you specify the clone? We have been using mAbs against p16alpha from
NeoMarkers (clone DCS 50.2) with little success and from Pharmingen (clone
G175-405; working conc. 5 ug Ig/ml, HIER in citrate buffer pH 6.0, pressure
cooker) with a little more success. While p16alpha is considered to be a
nuclear antigen, it seems that reactivity in the cytoplasm can occur (see
e.g. Gugger et al. 2001, Lung Cancer 33:229-39; significance?) - this is
probably what you identified as background.
Institute of Pathology, University of Bern, Switzerland
----- Original Message -----
From: "Robin Thain"
Sent: Friday, September 20, 2002 11:34 PM
Subject: p16 immuno staining
> Is anyone using p16? We have been trying to get this immuno stain up and
> running. Encountering lots of problems with background. This is a
> monoclonal antibody. TIA.
> Robin Stocks
> University of Alberta
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