Could you specify the clone? We have been using mAbs against p16alpha from
NeoMarkers (clone DCS 50.2) with little success and from Pharmingen (clone
G175-405; working conc. 5 ug Ig/ml, HIER in citrate buffer pH 6.0, pressure
cooker) with a little more success. While p16alpha is considered to be a
nuclear antigen, it seems that reactivity in the cytoplasm can occur (see
e.g. Gugger et al. 2001, Lung Cancer 33:229-39; significance?) - this is
probably what you identified as background.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland


----- Original Message -----
From: "Robin Thain" 
To: "Histonet" 
Sent: Friday, September 20, 2002 11:34 PM
Subject: p16 immuno staining


> Is anyone using p16?  We have been trying to get this immuno stain up and
> running.  Encountering lots of problems with background.  This is a
> monoclonal antibody.  TIA.
>
> Robin Stocks
>
> University of Alberta
>
>
>