Re: mounting media for double labeling

From:Heather Marie Hyland


Susan,
I use DPX Mountant (Fluka Biochemika 44581) to mount Cy2, Cy3, Cy5 triple
label slides.

Heather Ho
Clinical Lab Coordinator
Medical College of Wisconsin

On Wed, 25 Sep 2002, Susan Travers wrote:

> does anybody know of a mounting media compatible with double labeling 
> using the fluorescent cyanine dyes (CY2 & CY3) and BCIP/NBT.  These 
> particular fluorescent dyes won't withstand the anti-fading agent 
> phenylendiamine; the BCIP/NBT is compromised by xylene-miscible 
> mountants like permount and cytoseal.
> 
> Any ideas?
> 
> Thanks,
> Susan Travers
> Susan Travers, Ph.D.
> Associate Professor
> Ohio State University
> College of Dentistry
> Section of Oral Biology
> 305 West 12th Avenue #192
>   P.O. Box 182357
> Columbus, Ohio  43218-2357
> Tel: (614)292-6366
> Fax:614-247-6945
> email: travers.3@osu.edu
> 
> 




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