Re: mounting media for double labeling
|From:||Heather Marie Hyland |
I use DPX Mountant (Fluka Biochemika 44581) to mount Cy2, Cy3, Cy5 triple
Clinical Lab Coordinator
Medical College of Wisconsin
On Wed, 25 Sep 2002, Susan Travers wrote:
> does anybody know of a mounting media compatible with double labeling
> using the fluorescent cyanine dyes (CY2 & CY3) and BCIP/NBT. These
> particular fluorescent dyes won't withstand the anti-fading agent
> phenylendiamine; the BCIP/NBT is compromised by xylene-miscible
> mountants like permount and cytoseal.
> Any ideas?
> Susan Travers
> Susan Travers, Ph.D.
> Associate Professor
> Ohio State University
> College of Dentistry
> Section of Oral Biology
> 305 West 12th Avenue #192
> P.O. Box 182357
> Columbus, Ohio 43218-2357
> Tel: (614)292-6366
> email: email@example.com
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