Re: This is a stretch.....

From:

Geoff McAuliffe

The "standard" way to extract lipids is a 1:1 mixture of methanol and chloroform, used warm or hot. I forget the exact temperature and time but 30-60 minutes at 37C should do it. Do use a fume hood and NO open flames.

Geoff

Luis Chiriboga wrote:

Hi all.....but figured I would throw it out as a question. Does anyone know of a method to "extract" lipids (mainly traicylglycerols and cholesterol esters) from formalin fixed sections. --------------------------------------

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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