Re: This is a stretch.....

From:Geoff McAuliffe

    The "standard" way to extract lipids is a 1:1 mixture of methanol and chloroform, used warm or hot. I forget the exact temperature and time but 30-60 minutes at 37C should do it. Do use a fume hood and NO open flames.


Luis Chiriboga wrote:

 Hi all.....but figured I would throw it out as a question.   Does anyone know of a method to "extract" lipids  (mainly traicylglycerols and cholesterol esters) from formalin fixed sections. --------------------------------------

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

<< Previous Message | Next Message >>