Re: IHC problem: secondary reaction against sheep

From:Jan Shivers

Vector Laboratories makes 2 biotinylated anti-sheep IgG products (catalog #
BA-6000 and BA6080).  You can use their tertiary AB complex after it, or any
of the other (strept)avidin/biotin-based detection systems from other
companies.  I use DAKO's LSAB2 streptavidin reagent in my protocol after the
Vector rabbit anti-sheep IgG.

Jan Shivers
Univ. of Minnesota Veterinary Diagnostic Lab
  ----- Original Message -----
  Cc: ;
  Sent: Thursday, September 26, 2002 2:43 AM
  Subject: IHC problem: secondary reaction against sheep

  Dear histonetters,

  I need your help again. I'm not experienced in IHC.

  Unfortunately, I must to test an antibody against pepsinogen type II from
  My problem regards the secondary reaction: as far as I know, there aren't
kits to develop the secondary reaction against an antibody from sheep and it
could be become complicated to set up every single step of the secondary

  What shall I do? Does anybody know if there is a kit to resolve this

  Thanks in advance

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