Re: IHC problem: secondary reaction against sheep
> My problem regards the secondary reaction: as far as I know, there
> aren't kits to develop the secondary reaction against an antibody from
> sheep and it could be become complicated to set up every single step of > the secondary reaction.
> What shall I do? Does anybody know if there is a kit to resolve this
You can use some antibody detection kits for goat Ig, which bind well to
sheep antobodies. Sigma (to mention just an example) sells a kit based
in avidin/biotin and also the monoclonal anti goat/sheep IgG antibody,
labelled in many ways.
Another option is to use "universal detection kits based on recombinant
Protein G, but the binding to sheep Ig is a little on the weak side.
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