Re: Heart Stain
You are probably thinking of the gross succinnic dehydrogenase histochemical
enzyme technique. Undamaged heart has a positive reaction. Infarcted areas
have no reaction. The colour depends on the tetrazolium salt you use. I
used to use the cheapest I could find, which gave a dull red result. You
need quite a volume of the working solution, enough to colour a whole slice
of the tissue. The solution consists of sodium succinate, buffer and
tetrazolium salt. No co-enzyme is required. The success of the method
does, of course, depend on using fairly fresh heart.
I am not at home at the moment. Contact me again in 10 days or so if you
want me to dig out the details.
----- Original Message -----
From: "Jason Fowler"
To: ; ;
Sent: Thursday, October 03, 2002 8:54 AM
Subject: Heart Stain
> Is anyone familiar with a stain used for post-mortem hearts to determine
> areas of infarction? I am vaguely familiar. I think the stain marks
> areas of infarction brown and the remaining tissue gray? Or vice versa?
> Any help would be fantastic.
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