Re:Dysferlin IHC

From:Jan Bauer

Such a coincidence. Last week I performed  some stainings with the Hamlet-1 
ab from novocastra on cryostat mouse tissues (including SJl which should 
have less) and on paraffin (rat, human). in none of these tissues I found 
specific staining of muscle cell membranes. What I found however on rat 
paraffin tissue was a strong selective staining of lymphoid endothelium and 
less strong staining on normal small vessel endothelium. To me this is 
again a Novocastra antibody which does not perform as it is supposed to 
(anyone ever tried the FasL antibody and found a REAL specific staining?).

Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628

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