Re: Dysferlin IHC

From:"R.Z. Meitus"

I have been using a 1:20 dilution.  It seems to "work"
but I have found that you don't get the same crisp
sarcolemmal labeling as you do with some of the others
(e.g. dystrophin).  

--- "Mitchell (Jean)" 
> Can anyone shed some light on working with a
> dysferlin antibody?  
> I am working with NCL-Hamlet (Novocastra) and having
> a devil of a 
> time getting any dilution to work on normal frozen
> muscle.
> I have contacted the company as well but would
> appreciate any 
> expertise anyone could forward my way.
> Thanks much,
> Jean Mitchell, BS, HT (ASCP)
> University of Wisconsin Hospital & Clinics
> Neuromuscular Laboratory
> Madison, WI

Rebecca Z. Meitus, HTL
Vanderbilt University Medical Center
Department of Pathology
Division of Neuropathology

Do you Yahoo!?
Yahoo! News - Today's headlines

<< Previous Message | Next Message >>