Jean,
I have been using a 1:20 dilution.  It seems to "work"
but I have found that you don't get the same crisp
sarcolemmal labeling as you do with some of the others
(e.g. dystrophin).  

--- "Mitchell (Jean)" 
wrote:
> Can anyone shed some light on working with a
> dysferlin antibody?  
> I am working with NCL-Hamlet (Novocastra) and having
> a devil of a 
> time getting any dilution to work on normal frozen
> muscle.
> 
> I have contacted the company as well but would
> appreciate any 
> expertise anyone could forward my way.
> 
> Thanks much,
> 
> Jean Mitchell, BS, HT (ASCP)
> University of Wisconsin Hospital & Clinics
> Neuromuscular Laboratory
> Madison, WI
> 



=====
Rebecca Z. Meitus, HTL
Vanderbilt University Medical Center
Department of Pathology
Division of Neuropathology

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