I have used:
		70ml 95% EtOH
		20ml Glycerol
		 5ml HOH, to prepare 70% EtOH that is 20% glycerol.  Have
tissues that have been fine for 20 years as long as I properly seal the vial
(I use a dip in melted paraffin).

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Geology-Astronomy
West Chester University of Pennsylvania
Schmucker Science Center II
South Church street & Rosedale Avenue
West Chester, PA, 19383, USA
Phone:  610-738-0437
FAX:  610-738-0437
eMail:  fmonson@wcupa.edu
http://darwin.wcupa.edu/casi/
http://www.wcupa.edu/_visitors/


-----Original Message-----
From: Rita Angel [mailto:RITA.ANGEL@UC.EDU]
Sent: Thursday, September 26, 2002 1:24 PM
To: histonet@pathology.swmed.edu
Subject: storage of tissues


Hi all,
I have a question about storage of tissue that I hope someone can help me 
with. Does anyone know how long animal tissues can be stored in 70% ethanol 
without any damaging effects or hardening of tissues (after they've been 
fixed)?
We normally don't keep our tissues in 70% ethanol any longer than a few 
weeks, but we've been told we need to keep all of our formalin under a fume 
hood, (even if they're in closed containers) which we have very limited 
space for. These are tissues that we want to keep long term; we dispose of 
unwanted tissue 30 days after we receive them. If we could store them in 
ethanol, this would solve our problem.
Thanks for all of your help in advance.

Rita Angel