RE: some general questions on the TUNEL assay

From:=?iso-8859-1?Q?Martin_Beno=EEt?=

Could you explain me the TUNEL method ?


Benoit MARTIN
 Bruxelles


> -----Message d'origine-----
> De:	Amanda Gifford [SMTP:agifford@ctl.net]
> Date:	mercredi 18 septembre 2002 20:29
> :	histonet@pathology.swmed.edu
> Objet:	RE: some general questions on the TUNEL assay
> 
>  
> 
> 	I have gotten consistent results with TUNEL without working DNase
> free.  I have never tried it on decalcified tissue, but I'm not aware of
> problems with EDTA.  High concentrations of HCl would affect the DNA, but
> I doubt the concentration in the decalcifying solution  would affect the
> results of TUNEL.  Again though, I have not tried it.
> 	 
> 	Amanda Gifford, HTL
> 	CTL Immunotherapies
> 	agifford@ctl.net
> 



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