I have gotten consistent results with TUNEL without working DNase free.  I have never tried it on decalcified tissue, but I'm not aware of problems with EDTA.  High concentrations of HCl would affect the DNA, but I doubt the concentration in the decalcifying solution  would affect the results of TUNEL.  Again though, I have not tried it.

 

Amanda Gifford, HTL

CTL Immunotherapies

agifford@ctl.net