RE: Processing Carnoy's Fixed Tissue
Is it possible to post a photomicrograph onto the www.histonet.org website? I'd really like to see what your nuclei look like. It could be the "cornflaking" artifact that you get when you have sections that dry before coverslipping.
Initially my first thought is that you do not need to transfer to 70% alcohol prior to processing. It's an anhydrous fixative, and therefore should probably stay anhydrous through holding and processing. Otherwise, more information about your processing schedule and reagents would also be helpful.
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
From: ncragg [mailto:firstname.lastname@example.org]
Sent: Wednesday, September 18, 2002 3:24 AM
To: 'HistoNet Server'
Subject: Processing Carnoy's Fixed Tissue
I'm emailing from Manchester in the UK and just starting my histology
career. Please can anyone help?
Just read some archived mail on Histonet regarding processing carnoy's
fixed tissue - I really need some help with this, as we are getting strange
black/brown nuclei throughout the tissue. It has been suggested that this
is the acid remaining in the nuclei. We have a Leica carousel tissue
processor without a vacuum, however, I have used an overnight 18 hours
program and still can't get rid of this. We also use other fixatives which
aren't a problem, but we would like to resolve the problem with carnoy's
fixed tissue, as we use this fixative a lot in our lab and it is necessary
for soem of our work. Tissues are fixed for approx. 1 hour in Carnoy's and
then transferred to 70% prior to processing.
I have also read on Histonet that there are 2 references regarding the
processing of carnoy's fixed tissue, however, there aren't any abstracts on
PubMed and I'm not sure whether our library holds this journal. Does
anyone know how I can get hold of them? They are:-
Puchtler H et al Histochemie 16:361-371, 1968
Puchtler H et al Histochemie 21:97-116, 1970
If anyone has any other advice for dealing with these tissues, I would be
very grateful if you could pass it on.
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