Amy,
     
     We use both PSA and PSAP from Biocare Medical, detection with
Envision 
     (Dako), and get the cleanest prostate preparations the pathologists

     have ever seen.
     If you need any further info, don't hesitate to ask
     
     Ronnie Houston
     Regional Histology Operations Manager
     Bon Secours HealthPartners Laboratories
     5801 Bremo Road
     Richmond, VA 23226


______________________________ Reply Separator
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Subject: PSA antibody dilution
Author:  "Amy Johnson"  at MSGHub
Date:    10/1/02 4:23 PM


Hello histonetters,
 Our lab is having a problem with our PSA antibody.  We currently have
it
diluted to 1:2000 and we have lots of background staining and it just
looks 
terrible.  The method of immuno staining we do is the LSAB method.  We
get 
our antibody from Dako and the spec sheet says the  dilution should be
1:100 
to 1:200 with the PAP method of staining.  Do we need to use a different

method or different antibody???  We use a piece of prostate for the
control, 
should we use a different type of tissue for a control??   Any help
would be 
appreciated.  Thanks in advance.
     
     
Amy Johnson
Associates in Pathology
Wausau Wisconsin