RE: PSA antibody dilution

From:"Dawson, Glen"


I use the same antibody.  With the LSAB2 system, try it at a 1:10,000
dilution.  Stick with the LSAB2 system as the PAP method is less sensitive
and you'll waste antibody.

Good Luck,

Glen A. Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: Amy Johnson []
Sent: Tuesday, October 01, 2002 3:24 PM
To: Histonet
Subject: PSA antibody dilution

Hello histonetters,
 Our lab is having a problem with our PSA antibody.  We currently have it
diluted to 1:2000 and we have lots of background staining and it just looks
terrible.  The method of immuno staining we do is the LSAB method.  We get
our antibody from Dako and the spec sheet says the  dilution should be 1:100
to 1:200 with the PAP method of staining.  Do we need to use a different
method or different antibody???  We use a piece of prostate for the control,
should we use a different type of tissue for a control??   Any help would be
appreciated.  Thanks in advance.

Amy Johnson
Associates in Pathology
Wausau Wisconsin

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