There are several enzyme histochemical methods that could be used, 
     depending how soon after death the hearts were to be examined.
     One method I recall uses a gel technique that will detect an 
     endogenous glycogen-dependent phosphorylase even after 24 hr. I'll 
     need to search through my notes/references if you are interested. 
     
     Ronnie Houston
     Regional Histology Operations Manager
     Bon Secours HealthPartners Laboratories
     5801 Bremo Road
     Richmond, VA 23226


______________________________ Reply Separator
_________________________________
Subject: RE: Heart Stain
Author:  "Sebree Linda A."  at MSGHub
Date:    10/3/02 2:53 PM


I believe you may be referring to C9, an immuno stain.  There used to be
a 
histochemical stain referred to as HBFP (hematoxylin, basic fuchsin,
picric 
acid) that was developed at Mayo.  It fell out of favor when it was
realized 
that the result could be manipulated by more or less decolorization.  C9

antibody is available from The Binding Site, Cat. # PH028. You will also
need 
their secondary Ab: biotin conjugated donkey anti-sheep/goat, Cat. #
AB360.  We 
use these on Ventana immunostainers at 1:50 for the C9 and 1:10 for the 
secondary.  Incubation is for 32" after HIER in citrate buffer.  Hope
this is 
what you had in mind.
     
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
     
     
-----Original Message-----
From: Jason Fowler [mailto:jfowler@hsc.wvu.edu] 
Sent: Thursday, October 03, 2002 10:54 AM
To: weber.326@osu.edu; histonet@pathology.swmed.edu; thoward@unm.edu 
Subject: Heart Stain
     
     
Is anyone familiar with a stain used for post-mortem hearts to determine

areas of infarction?  I am vaguely familiar.  I think the stain marks 
areas of infarction brown and the remaining tissue gray?  Or vice versa?
 Any help would be fantastic.
     
Thanks,
Jason