RE: Heart Stain
There are several enzyme histochemical methods that could be used,
depending how soon after death the hearts were to be examined.
One method I recall uses a gel technique that will detect an
endogenous glycogen-dependent phosphorylase even after 24 hr. I'll
need to search through my notes/references if you are interested.
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
______________________________ Reply Separator
Subject: RE: Heart Stain
Author: "Sebree Linda A." at MSGHub
Date: 10/3/02 2:53 PM
I believe you may be referring to C9, an immuno stain. There used to be
histochemical stain referred to as HBFP (hematoxylin, basic fuchsin,
acid) that was developed at Mayo. It fell out of favor when it was
that the result could be manipulated by more or less decolorization. C9
antibody is available from The Binding Site, Cat. # PH028. You will also
their secondary Ab: biotin conjugated donkey anti-sheep/goat, Cat. #
use these on Ventana immunostainers at 1:50 for the C9 and 1:10 for the
secondary. Incubation is for 32" after HIER in citrate buffer. Hope
what you had in mind.
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: Jason Fowler [mailto:firstname.lastname@example.org]
Sent: Thursday, October 03, 2002 10:54 AM
To: email@example.com; firstname.lastname@example.org; email@example.com
Subject: Heart Stain
Is anyone familiar with a stain used for post-mortem hearts to determine
areas of infarction? I am vaguely familiar. I think the stain marks
areas of infarction brown and the remaining tissue gray? Or vice versa?
Any help would be fantastic.
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