RE: Heart Stain

From:"Sebree Linda A."

I believe you may be referring to C9, an immuno stain.  There used to be a histochemical stain referred to as HBFP (hematoxylin, basic fuchsin, picric acid) that was developed at Mayo.  It fell out of favor when it was realized that the result could be manipulated by more or less decolorization.  C9 antibody is available from The Binding Site, Cat. # PH028. You will also need their secondary Ab: biotin conjugated donkey anti-sheep/goat, Cat. # AB360.  We use these on Ventana immunostainers at 1:50 for the C9 and 1:10 for the secondary.  Incubation is for 32" after HIER in citrate buffer.  Hope this is what you had in mind.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
From: Jason Fowler []
Sent: Thursday, October 03, 2002 10:54 AM
Subject: Heart Stain

Is anyone familiar with a stain used for post-mortem hearts to determine
areas of infarction?  I am vaguely familiar.  I think the stain marks
areas of infarction brown and the remaining tissue gray?  Or vice versa?
 Any help would be fantastic.


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