RE: Embedding

From:"Gladney, Diane C Ms MACH"


I agree with Jackie. This is the way that I have always embedded. I train
our military med. techs. that are assigned to me to embed this way, too. I
have been a histotechnologist for 26 years and have worked in many labs.
This is the way that embedding has been done at the labs that I have worked
(my husband was prior military so we have lived all over the world). Is the
pathologist(s) telling you that you are embedding wrong? If it is, then he
really needs and education in histotechnological techniques. I may have been
trained in the "old school" but I believe that this is the way that it is
still being taught. Good luck and stick by your training.


-----Original Message-----
From: Jackie.O' [mailto:Jackie.O']
Sent: Thursday, September 19, 2002 3:06 PM
To: Nita Searcy
Subject: Re: Embedding

Nita -
I worked in a derm lab (for Surgipath years ago) and found the best way to
embed skin was just as you described - so you don't hit one hard piece of
epithelium and chip out your whole "straight' line.  I've trained histotechs
to embed this way as well, with all tissues, not just skin.  The denser or
harder area of the tissue always is the last to pass the knife edge so you
section the softer areas first, avoiding dragging cutting artifact from hard
tissue and tearing through the soft.  Does that make sense to you guys?

Jackie O'Connor
Global Pharmaceutical Research and Development
Abbott Laboratories
Drug Discovery -Chemotheraputics
Abbott Park, IL  60064


                    Nita Searcy


                                         Subject:     Embedding


                    01:26 PM



I have a question regarding "proper embedding"---I have been practicing for
yrs., am at new position and funny thing, I do things incorrectly---

Anyway, correct embedding--epithelium from skin - "all going same way" but I
embed on a slant not  in a straight row. The knife blade hits one piece at a
time not the entire
"straight " line.

Glenda Hoye and the instructors- do you have a reference?

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