RE: ER beta
I have used ER beta antibody (from Serotec, Inc., Cat. # MCA1974S) on formalin FFPE non-human primate mammary gland without any problems. I do HIER using Citra buffer, pH 6.0, for 45 minutes in a steamer, followed by a cool down period for 20 minutes. I then perform our standard procedure using the BioGenex SuperSensitive alk. phos. detection kit. I will send you my complete protocol via an attachment in a separate e-mail.
From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu]
Sent: Wednesday, September 18, 2002 9:12 AM
Subject: ER beta
Has anyone had any success with ER beta on formalin fixed paraffin embedded tissue sections. I saw one post in the archives but it did not give the details.
Any info would help like retrieval methods, incubation times.
Shands Teaching Hospital
352-265-0111 ext 72114
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