RE: ER beta

From:Hermina Borgerink

Hi Elaine,

I have used ER beta antibody (from Serotec, Inc., Cat. # MCA1974S) on formalin FFPE non-human primate mammary  gland without any problems.  I do HIER using Citra buffer, pH 6.0, for 45 minutes in a steamer, followed by a cool down period for 20 minutes.  I then perform our standard procedure using the BioGenex SuperSensitive alk. phos. detection kit.  I will send you my complete protocol via an attachment in a separate e-mail.


-----Original Message-----
From: Elaine Dooley [] 
Sent: Wednesday, September 18, 2002 9:12 AM
Subject: ER beta

Dear Histonetters,

Has anyone had any success with ER beta on formalin fixed paraffin embedded tissue sections.  I saw one post in the archives but it did not give the details.
Any info would help like retrieval methods,  incubation times.

Elaine Dooley
Shands Teaching Hospital
Gainesville FL
352-265-0111   ext 72114

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