RE: Calcification of Mouse Tissues

From:Tony Henwood

Von Kossa demonstrates phosphates and other "-ates" bound to calcium ie it replaces the calcium with silver. Unfortunately nuclear debris, rich in phosphates will also stain with silver (try a small cell lung carcinoma to see what I mean).
I would confirm the results with an alizarin red s or similar calcium chelating dye method.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Keith Rogers []
Sent: Tuesday, 24 September 2002 4:45
Subject: Calcification of Mouse Tissues

Dear Histonet,

I hope to catch you all before you head west to California.

Reviewing slides with an unusual pattern of calcium deposition, confirmed with Von Kossa, one of our pathologists asked if this could be a histology induced artefact. The calcium is deposited in all of the normal places, gut, kidney etc, and also in heart. Although calcium deposition in the heart is not uncommon, there seems to be no good explanation for it. i.e. there is no underlying condition which would explain the calcium deposits. What is more of a concern is the deposition of calcium inside the nuclei of some cells.

I would be grateful to hear from anyone who has experience with this or any thoughts on the subject.

Keith Rogers
NCI Cancer Research Center

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