RE: C4d staining for renal biopsies

From:"Sebree Linda A."


We only do C4d on frozen renal biopsies  mainly because the clinicians want their answer sooner rather than later.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
From: Martha Ward []
Sent: Friday, September 27, 2002 10:22 AM
Subject: C4d staining for renal biopsies

Our lab has had inquiries about adding this antibody to our protocol for transplant renal biopsies and I wanted to hear from labs that are currently performing it.  All the protocols I have seen reference frozen tissue but we were wondering if there is a way to do it on ffpe tissues.  Any and all help will be greatly appreciated!
Martha Ward, MT (ASCP) QIHC
Wake Forest University Baptist Medical Center
Winston-Salem, NC

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