From:"Monson, Frederick C."

Looked everywhere on my shelves, Allen.  Didn't find anything until I went to the OLD stains book.
Lillie, RD(1950), Anat Rec., 108:239-253.  Cited in Conn's 9th ed, by RD himself.
The subject of the paper was the PAS, and I'm not certain how the Methylene Green was used.

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University of Pennsylvania
Schmucker Science Center II
South Church street & Rosedale Avenue
West Chester, PA, 19383, USA
Phone:  610-738-0437
FAX:  610-738-0437

-----Original Message-----
From: Smith, Allen []
Sent: Monday, September 23, 2002 12:16 PM

Several books say that methylene green (the nitro analog of methylene blue; not methyl green) can be used as a nuclear stain, but give no procedure.

Does anyone in histoland have a protocol for methylene green staining of nuclei?

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

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