Immunos on mouse tissues
I have some mouse tissues (placentas, embryos mainly) I am trying to do an
immuno with an antibody raised in goat. I am using Sigma's Extravidin kit
and noticed their anti-goat biotin was raised in mouse. Is this going to
cause me problems? I have successfully used mouse primary on mouse tissues,
but never tried a secondary.
A second question; do people recommend doing antigen retrieval? I tried
doing pepsin digestion and I got no signal in my immunos.
Your input would be much appreciated.
Columbus Children's Hospital
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