Cyclin D1
Hello again,
We are having problems getting a consistent stain with Cyclin D1. Does anyone have any ideas?
Procedure:
Steam retrieval in EDTA for 40 minutes
Cool for 30 minutes
Primary for 45 minutes
Link for 20 minutes
Strep for 20 minutes
DAB for 5 minutes
Hema for 5 seconds
Blue
Coverslip
This procedure is ran on the DAKO Autostainer. Any suggestions would be appreciated. Thank you.
Jim Bradford
ORMC
Orlando, FL
<< Previous Message | Next Message >>