To perform immunohistochemistry in chicken tissues:

1. What is the best blocking reagent? A solution containing 3-5% of the
serum of the secondary antibody host, 1-3% BSA, 3% glycine or purified
casein?

2. What is the best biotinylated secondary antibody? It can (must) be from
chicken or from a different species?

Regards

* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Apartado 94
7002-554 ...vora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs@uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm