Carnoy's fixed tissue - Photomicrographs and Method
Thank you for all your replies - I am very grateful and hopefully I will
be able resolve this problem. I have attempted to post a couple of
photomicrographs on the website to demonstrate the nuclei;
tonguecarnoys.jpg & gutcarnoys.jpg It may not be as good quality as you
are used to as I have only been practicing histology for a few weeks! The
photomicrographs don't look exactly like what you see down the microscope,
but this is also the first time I've used the Spot Advance Camera!!
The protocol used for the tissue shown in the photomicrographs was as
Fix in carnoy's for approx 1 hour, then transfer to 70% before processing.
1 70 30 minutes
2 90 30 minutes
3 95 30 minutes
4 100 1 hour
5 100 1 hour
6 100 2 hours
7 100 2 hours
8 Xylene 1 hour
9 Xylene 1 hour
10 Xylene 2 hours
11 Wax 2 hours 30 minutes
12 Wax 4 hours
Initially I used shorter programs, but on finding these black/brown nuclei,
we decided to increase the length of time in 100% and Xylene to see if that
would resolve it, which is how we arrived at the protocol above.
I am going to try rinsing in 95% or 100% before processing as Gayle
FAO: Terry Marshall - Nice to hear from someone over this side of the
pond!! I will post you a slide if that helps!?
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