blocking question

From:"Hawkins, Hal K."

We often have problems in immunostaining the trachea and bronchi
in the sheep, with nonspecific binding of rabbit antibody onto mucus and 
cartilage.  I suspect this is charge-related.  Does anyone have 
experience with a blocking step that will reduce this problem,
perhaps by blocking the strong anions on mucus and cartilage?


Hal Hawkins
UTMB, Galveston, Texas

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