apoptosis- TUNEL vs cleaved caspase 3

From:"L. Muffley"

Hello -
There is an excellent article in the Journal of
Histochemistry and Cytochemistry Vol 50(4): 449-454, 2002
recommending the use of Cleaved Caspase 3 antibody to detect apoptosis
(TUNEL assay method can also grab fragmented DNA of necrotic cells).
We have the antibody to cleaved caspase 3 on order and will try it in our
lab soon.


				Lara Muffley
                                Dermatology Dept
                                University of Washington
                                Seattle, WA
                                muffley@u.washington.edu




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