SV: Daily Digest


Denne meldingen inneholder ikke sensitiv informasjon som bryter med
Rikshospitalets krav til informasjonssikkerhet.
(In compliance with the Security Policy of Rikshospitalet , the content
of this message (including any attachments) is of a non-sensitive

Hi Jen,
The enzyme HRPO will not bind Fluorophore-conjugated biotin.
You should try TSA (Tyramide Signal Amplification) where HRPO is used to
catalyse deposition of fluorophore labeled tyramide.
You may also try fluorophore labeled anti-mouse or anti-rabbit (depending on
your primary) It may work eaven thoug your primary is HRPO labeled.
Wish you all welcome to Immunohistochemistry - In Situ Hybridization at 

Hogne Roed Nilsen

Date: 12 Sep 2002 19:15:58 -0500
From: "Philopena, Jennifer" 
Subject: converting a chromogen signal to fluorescence

Hi All.
I have an HRP-conjugated primary antibody that works very well for IHC (and
I'd like to keep using it), but I want a fluorescent signal.  Does anyone
know of a fluor-conjugated anti-HRP antibody?  If not, does anyone think it
will work if I use fluor-conjugated biotin (the biotin will hopefully bind
without background to the HRP, and I'll have a fluorescing signal)?
Thanks for your insight.

 This message and any attachments is solely for the intended recipient. If
you are not the intended recipient, disclosure, copying, use, or
distribution of the information included in this message is prohibited --
please immediately and permanently delete this message.

<< Previous Message | Next Message >>