Thank you very much for the reply!
I forgot to mention in my original message that the reason we want to
detect zinc is to estimate the volume of the mossy fiber system in the
hippocampus. I am not very familiar with SEM techniques, but I suppose
we cannot examine sections as large as a full hippocampal coronal
section under the SEM.
Is that true?
Is there any other way to stain Mossy fiber system in tissue  already
processed for EM?

Thank you in advance.

Despina A. Tata
Biological Psychology
Dept. of Psychology
S.U.N.Y. Stony Brook

> Hi Despina,
>
> X-ray microanalysis would be the easiest answer. If you have an SEM
> with X-ray microanalysis and a backscatter detector system, you could
> examine semi-thins mounted onto plastic film carbon coated as for a
> scanning sample.
>
> Cheers
>
> Steve
>
>
>
>
>                     dtata
>
>                      histonet@pathology.swmed.edu
>                     ysb.edu>             cc:
>
>                                          Subject:     zinc detection
>
>                     04/09/2002
>
>                     17:14
>
>
>
>
>
>
>
>
> Hi all,
> I was just wondering if anyone knows whether tissue processed for
> electron microscopy (osmicated and embedded in epoxy) can be further
> processed for zinc detection.
> Thanks!
>
> Despina
>
>
>
>