Re: uneven IHC staining patterns

From:Richard Cartun

Is your problem with all your antibodies or only a few?  Do you see it
more often with tissues that contain an abundance of the antigen that
your are staining for?

Rich Cartun

>>> Pat Zeitlow  09/10/02 12:50PM >>>
We are periodically getting uneven staining patterns on our IHC
I have exhausted my troubleshooting skills and need input from anyone
out there who may be able to help.  We are using the Dako Autostainer
different ones).  I have checked the leveling, reagent distribution,
slide position.  I am in a reference laboratory and unable to do much
terms of trouble shooting fixation, etc.   I am at wit's end and will
happily provide any other details to assist.  Dako says they are
clueless and have heard no other similar complaints.  Please help. 
Pat Zeitlow
Molecular Pathology Manager
Boyce and Bynum Laboratories

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