Re: hydroxyapatite

From:louise renton

Dear Kathleen

We decalcify our research tissue implants in a formic acid/HCl decalcifier 
(in house recipe) prior to processing for paraffin wax. I do not know the 
composition of our implants (work done before my time), but they appear to 
have a residual structure that remains in the tissue and can be seen when 
staining. You could also try surface decalcifiaction of your blocks. Face 
the block(trim) and then place face down in a coplin jar lid with a little 
of your favourite brand of decal fluid - leave for a while, and then try 
setioning again.

ref: Journal of Bone &Joint Surgery Vol83-A: Supplement 1 part 2 -2001
Bone induction by BMPs/OPs and related family members in primates. etc

Best Regards

Louise Renton
Bone Research Unit
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana

>From: Kathleen Cormier To: Subject: 
>hydroxyapatite Date: Tue, 03 Sep 2002 13:02:13 -0400
>Hello all,
>I have a researcher who is submitting hydroxyapatite and cells as FFPE. Is 
>it possible to get acceptable sections from hydroxyapatite that are FFPE 
>and not decalcified? All I get is shredding. The researcher is afraid that 
>decalcification would compromise the integrity of the construct. Any 
>suggestions or references would be greatly appreciated!
>Kathy Cormier Histology Supervisor Division of Comparative Medicine MIT

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