Re: Immuno on JB 4 Resin.
Hi, my experience is simple: unsuccessful immunohistochemistry using
JB4. We were not able to achieve any immunostaining using various
antibodies and lectins that work well in paraffin. We tried JB4 because
got some references saying that it was a good resin for immuno, but we
did not find any difference with our standard Historesin: the protocols
just didn't in neither of them. I would appreciate if somebody gives
protocols or tips to get good immunohistochemistry in resin.
Inst. Acuicultura Torre la Sal (CSIC)
12595 Ribera de Cabanes, Castellon
Melanie Black wrote:
> Hi There
> I am setting up JB4 resin, and have managed to get quite good Actin
> staining on JB 4 embedded tissue. Has anybody done immuno on this resin?
> I would be interested to discuss methods, counterstain, successful
> antibodies, etc.
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