Re: Alkaline Phosphatase for meningiomas
From memory, and that ain't so good now (no correlation with malt whisky!),
I had best success with one of the original metal salt methods of Gomori. I
was looking at CNS tumors in culture and did a wide variety of enzyme
From recollection, sodium beta-glycerophosphate was the substrate, with
calcium and magnesium chlorides in the incubation medium also. If you can't
get a hold of a copy of one of the editions of Pearse's Histochemistry, let
me know and I'll try to dig out the method.
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
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Subject: Alkaline Phosphatase for meningiomas
Author: "R.Z. Meitus" at BSHSIBTW
Date: 9/4/02 1:38 PM
I am not having much luck here. Does anyone have a
"tried and true" method/protocol that they wouldn't
Rebecca Z. Meitus, HTL
Vanderbilt University Medical Center
Department of Pathology
Division of Neuropathology
--- "R.Z. Meitus" wrote:
> Hello All,
> I have been asked by one of our neuropathologists to
> do an alk phos on frozen sections of meningiomas. I
> have tried 2 methods so far with no results.
> One method was
> (not the APAAP method);
> and the other was out of the Freida Carson text.
> I've searched the histonet archives and found that
> someone suggested using an alk phos kit (e.g.
> The vector catalog says that these kits are not
> recommended for staining neuro stuff.
> Any help will be greatly appreciated, esp. someone
> with a protocol.
> Thank you in advance,
> Rebecca Z. Meitus, HTL
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