Re: Acridine orange staining for lysosomes: Fixed tissue?

From:"J. A. Kiernan"

Kerenza Njoh wrote:
> ... has anybody used acridine orange staining to look at 
> lysosomes present in paraformaldehyde fixed tissue?

You wouldn't expect acridine orange to stain lysosomes
in fixed tissue, though it has been used for that purpose
with living cells (Manyonda,IT & Choy,MY 1999 J. Soc.
Gynec. Invest. 6:158-166). A traditional histochemical 
method for acid phosphatase activity should do the job 
for lightly fixed material, and you'll have the added 
advantage of a permanent preparation.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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