We had similar problems when our tubes dispensing the reagents were
partially clogged.  Some sections would get proper amount dispensed, others
got partial amount which did not cover the whole section with all reagents.
Dako came out and cleaned/replaced tubing and things got back to normal.

Karen Lahti, HT(ASCP), QIHC
Sonora Quest Laboratories

-----Original Message-----
From: Pat Zeitlow [mailto:pzeitlow@bbpllab.com]
Sent: Tuesday, September 10, 2002 9:51 AM
To: histonet@pathology.swmed.edu
Subject: uneven IHC staining patterns


We are periodically getting uneven staining patterns on our IHC slides.
I have exhausted my troubleshooting skills and need input from anyone
out there who may be able to help.  We are using the Dako Autostainer (2
different ones).  I have checked the leveling, reagent distribution, and
slide position.  I am in a reference laboratory and unable to do much in
terms of trouble shooting fixation, etc.   I am at wit's end and will
happily provide any other details to assist.  Dako says they are
clueless and have heard no other similar complaints.  Please help.  TIA.

Pat

Pat Zeitlow
Molecular Pathology Manager
Boyce and Bynum Laboratories
573-886-4600