RE: Safran du Gatinais
From: | "Monson, Frederick C." |
Morning Jenny,
Safran du Gatinais = Saffron of Gatinais
Saffron (Roboz)(C.I. #75100)* 6g (about 1/4 oz) (stigmas or
powder, I bet!)
100% Ethanol 100ml
saffron in EtOH in air-tight brown bottle*** (125ml serum
bottle with Teflon-lined screw cap)
leave at 56-58oC for 48hr**
stain for 15min in screw-cap Coplin jar (this is a last
counterstain)
The above came out of S.W. Thompson's, Selected Histochemical and
Histopathological Methods, Thomas, Springfield, IL, 1966, p 827-828 (Movat's
Pentachrome Stain).
The web is wonderful!!!
A reference to a method/protocol contact for Movat's Penta-stain
came from Steve Slap at:
http://www.histosearch.com/histonet/Feb01A/MovatsPentachromeStainA.html
* Listed in Lillie's, "Conn's biological Stains", don't know about the new
edition soon to come.
** This is quite obviously an extraction performed on stamens (called
"saffron threads") or powder. You could presume that C.I. 75100 is a
purified powder from a similar organic extraction.
*** I always keep a quart can of black paint in my lab in which I dip small
bottles that I wish to make light tight. There is a plastic coating which
is available at Home Depot that comes in other colors
Saffron can be purchased in original form or as a powder as explained here:
http://saffron.com/cons_guide.html
A general link library for dyes (as in textiles!! where it all started) can
be found here:
http://moas.atlantia.sca.org/topics/dyes.htm
Saffron is expensive ($36/oz) for cooking and exorbitant for staining at:
http://www.voigtglobal.com/cdi.htm (search )
So, after all of the intelligent stuff listed above, the search finally
produced a summary in a PDF file from Luna's " "Histo-Logic" at:
http://206.137.77.9/sakura/pdf/2n20472.pdf. He not only gave a
modified version of Movat but also the original source. Movat(1955),
A.M.A., Arch Path, 60:289-294 (which is also in Thompson's book).
We (Mom and I) have a snitched (rescued?) mortar and pestle to grind the
"threads" to powder for cooking. Only a little powder is required for
non-chef-like cooking, so the mortar and pestle hang out in the cupboard
most of the time.
Hope this helps, it was a nice exercise to start the day for me,
Cheers,
Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
c/o Geology/Astronomy
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
Phone: 610-738-0437
FAX: 610-738-0437
fmonson@wcupa.edu
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/
THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.
> ----------
> From: Jenny Molde
> Sent: Friday, September 13, 2002 4:01 AM
> To: histonet@pathology.swmed.edu
> Subject: Safran du Gatinais
>
> I would like to attempt the Movat pentachrome stain and was wondering
> if anyone out there could please give me some info on where to find
> this dye, Safran du Gatinais. I have searched quite a number of
> companies but no success on my side. Many thanks in advance.
>
> Jenny Molde
>
>
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