RE: Safran du Gatinais
|From:||"Monson, Frederick C." |
Safran du Gatinais = Saffron of Gatinais
Saffron (Roboz)(C.I. #75100)* 6g (about 1/4 oz) (stigmas or
powder, I bet!)
100% Ethanol 100ml
saffron in EtOH in air-tight brown bottle*** (125ml serum
bottle with Teflon-lined screw cap)
leave at 56-58oC for 48hr**
stain for 15min in screw-cap Coplin jar (this is a last
The above came out of S.W. Thompson's, Selected Histochemical and
Histopathological Methods, Thomas, Springfield, IL, 1966, p 827-828 (Movat's
The web is wonderful!!!
A reference to a method/protocol contact for Movat's Penta-stain
came from Steve Slap at:
* Listed in Lillie's, "Conn's biological Stains", don't know about the new
edition soon to come.
** This is quite obviously an extraction performed on stamens (called
"saffron threads") or powder. You could presume that C.I. 75100 is a
purified powder from a similar organic extraction.
*** I always keep a quart can of black paint in my lab in which I dip small
bottles that I wish to make light tight. There is a plastic coating which
is available at Home Depot that comes in other colors
Saffron can be purchased in original form or as a powder as explained here:
A general link library for dyes (as in textiles!! where it all started) can
be found here:
Saffron is expensive ($36/oz) for cooking and exorbitant for staining at:
http://www.voigtglobal.com/cdi.htm (search )
So, after all of the intelligent stuff listed above, the search finally
produced a summary in a PDF file from Luna's " "Histo-Logic" at:
http://184.108.40.206/sakura/pdf/2n20472.pdf. He not only gave a
modified version of Movat but also the original source. Movat(1955),
A.M.A., Arch Path, 60:289-294 (which is also in Thompson's book).
We (Mom and I) have a snitched (rescued?) mortar and pestle to grind the
"threads" to powder for cooking. Only a little powder is required for
non-chef-like cooking, so the mortar and pestle hang out in the cupboard
most of the time.
Hope this helps, it was a nice exercise to start the day for me,
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale Ave
West Chester, Pennsylvania, USA, 19383
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/
THINKING IS MUCH MORE DIFFICULT THAN MEMORIZING.
> From: Jenny Molde
> Sent: Friday, September 13, 2002 4:01 AM
> To: email@example.com
> Subject: Safran du Gatinais
> I would like to attempt the Movat pentachrome stain and was wondering
> if anyone out there could please give me some info on where to find
> this dye, Safran du Gatinais. I have searched quite a number of
> companies but no success on my side. Many thanks in advance.
> Jenny Molde
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