RE: Reference for this nerve stain???

From:Tony Henwood

RE: Reference for this nerve stain???

Shannon,
Similar to a Bodians, but instead of gelatine, Bodians uses an albumen silver complex.

Tony

-----Original Message-----
From: Klassen, Shannon SDH [mailto:klassens@sdh.sk.ca]
Sent: Wednesday, 11 September 2002 6:36
To: 'Tony Henwood'
Subject: RE: Reference for this nerve stain???


Bodian?
Shannon Klassen
IHC Tech
Saskatoon Health District
Saskatoon, Canada

> ----------
> From:         Tony Henwood[SMTP:AnthonyH@chw.edu.au]
> Sent:         Monday, September 09, 2002 1:32 AM
> To:   Histonet (E-mail)
> Subject:      Reference for this nerve stain???
>
> Hi all,
> I have found this technique in the archives of my department and was
> wondering whether anyone has heard of it. I don;t have any idea of where
> it originates.
>
> GELATINE SILVER FOR NERVE FIBRES
> Principle:
> Nerve fibres and axons have the ability to absorb silver ions that are
> then reduced by hydroquinone.  The fibres are further darkened by oxalic
> acid.  Selectivity is improved by including gelatine in the silver
> solution.
>
>
> Fixation:      10% buffered formalin.
> Microtomy:     Paraffin sections at 5um.
>                
> Reagents:              
>
> 1.      Impregnating solution - prepare just prior to use:
>         Distilled water                                 50ml
>         Silver Nitrate                                  0.1g
>         Gelatine                                        0.4g
>         Place at 60oC for 15 minutes, mixing twice.
>
> 2.      Reducing solution:
>         Distilled water                                 50ml
>         Sodium Sulphite                                 0.5g
>         Hydroquinone                                    0.1g
>         Mix, stand for 20 minutes before use.
>
> 3.      0.2% Gold Chloride
>
> 4.      2% Oxalic acid
>
> 5.      2% Sodium Thiosulphate.
> #012#Procedure:
>
> 1.      Dewax and hydrate sections to distilled water.
> 2.      Place in impregnating solution, 5 hours - overnight (60oC).
> 3.      Dip slide in distilled water once.
> 4.      Place in reducing solution, 2 hours.
> 5.      Running water, 10 minutes.
> 6.      Distilled water, 1 minute.
> 7.      Gold Chloride, 5 minutes.
> 8.      Distilled water, 1 minute.
> 9.      2% Oxalic Acid, 2 minutes or longer.
> 10.     Running water.
> 11.     2% Sodium Thiosulphate, 5 minutes.
> 12.     Wash in water, 5 minutes.
> 13.     Dehydrate, clear and mount.
>
>
> Results:
> Axons and nerve fibres          black
> Nuclei                          shades of grey
>
> Thanks,
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at  Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: (02) 9845 3306
> Fax: (02) 9845 3318
>
> http://www.histosearch.com/homepages/TonyHenwood/default.html
> http://us.geocities.com/tonyhenwoodau/index.html
>
>
>
>
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>



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This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
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