RE: Mouse lung processing

From:"Prodhan, Parthak,M.D."

Hi Histonetters,

I am new to this field of histology. I was hoping to get some help from the
group members for my queries.

1. How does one fix and embedd postnatal mouse lung tissue? Stress on
maintaining architecture of the alveoli. I have had problems in maintaing
architecture with frozen sections. Any suggestions are most welcome.

2. For immunoflorescence on mouse lung sections using paraffin sections. Does it
give better results as compared to frozen sections.

Thank you in advance

Parthak Prodhan
Pediatric Pulmonary Lab
MassGeneral Hospital for Children
Boston, MA

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