RE: CSF cytology preparation procedure

From:Tony Henwood

RE: CSF cytology preparation procedure

This is our method:


1. Label 2-4 sticky slides (dependent on sample volume).

2. Erect slides in cytocentrifuge.

3. Add 3-4 drops fluid per tube.

4. Centrifuge at 500rpm for 5 min.

5. Remove slides and fix half slides in 95% alcohol for 20 min.  Allow remaining slides to air dry for DQ and Giemsa staining.

-----Original Message-----
From: Donato, Elizabeth M []
Sent: Thursday, 5 September 2002 9:07
To: 'HistoNet Server'
Subject: CSF cytology preparation procedure

Hello histonetters,

I was wondering if there was someone who would kindly be willing to share
their standard procedure for preparing a cerebral spinal fluid for a
cytology evaluation?  Thank you in advance for your help!

Liz Donato
Fred Hutchinson Cancer Research Center

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