| From: | Tony Henwood |
Elizabeth,
This is our method:
CEREBROSPINAL FLUID
1. Label 2-4 sticky slides (dependent on sample volume).
2. Erect slides in cytocentrifuge.
3. Add 3-4 drops fluid per tube.
4. Centrifuge at 500rpm for 5 min.
5. Remove slides and fix half slides in 95% alcohol for 20 min. Allow remaining slides to air dry for DQ and Giemsa staining.
-----Original Message-----
From: Donato, Elizabeth M [mailto:edonato@fhcrc.org]
Sent: Thursday, 5 September 2002 9:07
To: 'HistoNet Server'
Subject: CSF cytology preparation procedure
Hello histonetters,
I was wondering if there was someone who would kindly be willing to share
their standard procedure for preparing a cerebral spinal fluid for a
cytology evaluation? Thank you in advance for your help!
Liz Donato
Fred Hutchinson Cancer Research Center
edonato@fhcrc.org
206.667.4501
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