RE: 500um - 1000um frozen sections

From:"Bright, Alan"

RE: 500um - 1000um frozen sections


Our Bright OTF5000 Cryostat fitted with specimen temperature control &  graduated knife feed is capable of sections in excess of 500 microns.  For section sizes above 50 X 70 mm we also manufacture our Bright 8250 Cryostat fitted with a Sledge Microtome

Best regards

Alan Bright

Bright Instrument Co. Ltd.
St. Margaret's Way
PE29 6EU

Tel: +44(0) 1480 454528/451980/451499
Fax:+44(0) 1480 456031
Web Site:

-----Original Message-----
From: kbrand []
Sent: 05 September 2002 13:10
To: HistoNet Server
Subject: 500um - 1000um frozen sections


Our experiments we wish to do on mouse brain requires cutting sections
of 500um thickness, or greater, frozen.

Ideally, the mouse brain would be removed from the brain case, embedded
in OCT (oriented for coronal sectioning), and immediately snap frozen in
liquid N2- followed by sectioning at around 500um or more.

Is there an instrument available which can perform this, perhaps a
'cryovibratome'? And if so, is this instrument available in The
Netherlands/Belgium or anywhere nearby?

The tissue will be analysed by DNA microarray, so freezing is a must,
but the thicker the sections (up to 1000um), the better.


[g'day Dr T.]
Karl Brand <>
Department of Cell Biology and Genetics
Erasmus University
Dr. Molewaterplein 50
3015 GE Rotterdam
ph +31/10 408 7457 fax +31/10 408 9468

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