Problems with Processed specimens
Dear Histonetters
I am writing to ask for some advice.
We have suddenly started having problems with our processing.
The blocks have become much more difficult to cut. The amount of nuclear detail
is very poor. The nuclei show no chromatin detail and have a ground glass
appearance. The staining is awful and far too red.
I think the problem relates to the processor, there seems to
be some peculiar blobs in the wax. I think these may be xylene and
alcohol.
There is some suggestion within the department that the
problem may be due to a bad batch of fixative. We are using bought in filled
specimen pots from TBS in the UK. Has anybody in the UK experienced problems
with pre-filled buffered formal saline specimen pots from TBS.
Does anybody have any answers?
I can send photos to anybody who can offer help.
Thanks in advance
David Muskett
Alder Hey Children's Hospital
Liverpool, UK
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