Mounting vibratome sections

From:Matt McElwee

Hello Histonetters.  I have a question about doing
immunohistochemistry(IHC) on vibratome sections.  We're using a 16%
gelatin, 30% sucrose embedding medium.  We'd like to be able to do IHC
on these sections, but, of course, they won't stay on the slide when you
put them in solution.  Does anyone know of an embedding medium you can
use that will stick to a slide, or failing that, an easy way to do IHC
on vibratome sections?  Thanks.
Matt 
 
Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648
mcelwee@surgery.wisc.edu



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