> There are records of successful subculture of TB from 10% Formalin after
> months or years, can't remember.
> 
> Fred Monson
> 
> ----------
> From: 	Jackie.O'Connor@abbott.com
> Sent: 	Tuesday, September 10, 2002 1:16 PM
> To: 	Carrie Kyle-Byrne
> Cc: 	'HistoNet@Pathology.swmed.edu'
> Subject: 	Re: Interesting point! formalin separation from tissues for
> disposal
> 
> 
> Like everyone else, I've separated formalin from tissues for disposal -
> HOWEVER - I've NEVER filtered the spent formalin to remove all traces of
> tissue fragments. How else would you ensure all traces of possible tissue
> fragments have been removed?  How is this different than separating body
> fluids from formalin?  I can't imagine how you guys are doing this.  Are
> we
> forgetting the characteristics of a good fixative - like kill, penetrate,
> denature protein, etc?  Anybody ever cultured their spent formalin?  Good
> luck.  I'm using strictly STF for mouse tumors now - no formalin - yay!  I
> sure don't miss the formalin migraines - but I still have my nasal polyps
> to
> remind me.
> 
> 
>  
> 
>                     Carrie
> 
>                     Kyle-Byrne           To:
> "'HistoNet@Pathology.swmed.edu'"                              
>                     
> 
>                     ixis.com>            cc:
> 
>                                          Subject:     Re: Interesting
> point! formalin separation from tissues  
>                     09/10/2002           for disposal
> 
>                     11:36 AM
> 
>  
> 
>  
> 
> 
> 
> 
> 
> just another reason to use the formalin neutralizing solutions and dump
> down
> the drain.....
> 
> ----- Original Message -----
> From: "Gayle Callis" 
> To: "Horn, Hazel V" ;
> 
> Sent: Tuesday, September 10, 2002 8:59 AM
> Subject: Interesting point! formalin separation from tissues for disposal
> 
> 
> > Vinnie,
> >
> > Hazel brings up a good point.  Next question, is what do you do with the
> > separate blood and lipid waste? Has your safety people thought of that?
> or
> > do you have a way to dispose of these in another manner?
> >
> > Maybe looking into formalin recycling would be a good choice, since you
> > have to do so much work to satisfy the safety people.  Then the only
> waste
> > you have to dispose of is what is left in bottom of recycling unit,
> small
> > by comparison to volumes of formalin.  Personally, I don't think one can
> > separate lipid out totally, it makes a colloidal solution (hope I am
> > thinking correctly here) and I am sure everyone has seen cloudy formalin
> > from very fatty tissue - and if you can't get it all out, what does your
> > safety people make you do then??
> >
> > Good luck
> >
> >
> > At 09:02 AM 9/10/02 -0500, you wrote:
> > >I don't know if I can offer a reasonable solution, but wouldn't these
> > >elements be harmless as they are fixed?   I don't understand their
> reasoning
> > >for this.   Is the waste company that hauls the spent formalin away
> > >suggesting this?    We have to pour ours off as well.
> > >I sure hope this isn't something that will come my way.    I'll be
> > >interested in replies to this thread.
> > >Hazel
> > >
> > >> -----Original Message-----
> > >> From: Vinnie Della Speranza [SMTP:dellav@musc.edu]
> > >> Sent: Tuesday, September 10, 2002 8:43 AM
> > >> To: histonet@pathology.swmed.edu
> > >> Subject: formalin separation from tissues for disposal
> > >>
> > >> I know that this topic has been discussed on the list numerous times
> > >> before however we are being faced with a slightly different slant
> that
> I
> > >> would welcome your input on.
> > >>
> > >> Our Safety dept. has become conerned that our waste formalin contains
> > >> blood and lipid that they feel must be separated from the formalin
> before
> > >> it can be carted away.
> > >> We currently use a filtering funnel when pouring off the waste
> formalin
> > >> but this won't extract the lipid and blood which are in liquid
> suspension
> > >> in the formalin.
> > >>
> > >> Has anyone else been required to address this issue? I'm hoping
> someone
> > >> can offer a reasonable solution that will satisfy our Safety folks.
> > >>
> > >> thanks
> > >>
> > >>
> > >>
> > >> Vinnie Della Speranza
> > >> Manager for Anatomic Pathology Services
> > >> Medical University of South Carolina
> > >> 165 Ashley Avenue  Suite 309
> > >> Charleston, SC 29425
> > >> Ph: 843-792-6353
> > >> fax: 843-792-8974
> > >
> > >
> > >
> > Gayle Callis
> > MT,HT,HTL(ASCP)
> > Research Histopathology Supervisor
> > Veterinary Molecular Biology - Marsh Lab
> > Montana State University - Bozeman
> > 19th and Lincoln St
> > Bozeman MT 59717-3610
> >
> > 406 994-6367 (lab with voice mail)
> > 406 994-4303 (FAX)
> >
> > email: gcallis@montana.edu
> >
> >
> 
> 
> 
> 
> 
> 
> 
> 
>