Alkaline Phosphatase for meningiomas

From:"R.Z. Meitus"

Hello again,
I am not having much luck here.  Does anyone have a
"tried and true" method/protocol that they wouldn't
mind sharing?  

Rebecca Z. Meitus, HTL
Vanderbilt University Medical Center
Department of Pathology
Division of Neuropathology



--- "R.Z. Meitus"  wrote:
> Hello All,
> 
> I have been asked by one of our neuropathologists to
> do an alk phos on frozen sections of meningiomas.  I
> have tried 2 methods so far with no results.
> One method was
> http://www.nottingham.ac.uk/pathology/default.html
> (not the APAAP method);
> and the other was out of the Freida Carson text.
> I've searched the histonet archives and found that
> someone suggested using an alk phos kit (e.g.
> Vector).
> The vector catalog says that these kits are not
> recommended for staining neuro stuff.
> Any help will be greatly appreciated, esp. someone
> with a protocol.
> 
> Thank you in advance,
> Rebecca Z. Meitus, HTL
> 
> 
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