Dear Histonetters,

I am looking for a marker for hepatocytes in pig fetuses for use on frozen 
cryosections (immunoflourescence). I have previously used a commercial 
marker (hepatocyte specific antigen) with minimal success. In my recent 
stainings I noticed that streptavidin-FITC by itself appears to label only 
hepatocytes giving a distinct granular cytoplasmic stain. Is this an 
acceptable marker? Can anyone suggest a reference on endogenous biotin in 
fetal hepatocytes?

Many thanks in advance.

Romeo Sanchez (Philippines)
PhD student
University of Ghent, Belgium

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