epifluorescence visualization with pmma

From:Linda Jenkins

Hi, John!
	I have not encountered any problems when using bone labels (i.e., xylenol, 
calcein, tetracycline, etc.) and PMMA.  I would wonder if your post doc has 
the right filter(s) on the microscope to visualize the dyes?  Each label 
visualizes at a certain wave length.  This info should be in the catalog 
from which the dyes were ordered.  And, of course, the prepared slides 
should be stored in the dark as light will fade the labels.  Another 
thought - you might re-check the dosage just to make sure the right amount 
was administered.
	Hope this helps - off to Charlotte - be back Oct.1 if you need more info,
	Linda





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