cytology spray fixative and immunohistochemistry

From:"Corazon D. Bucana"

Hi All,

I was wondering if anyone has performed immunohistochemistry on cells such
as buccal mucosa smears fixed with a cytology spray fixative?  If so, what
antibodies did you use?   I assume the spray fixative contains some type of
alcohol.  We are planning to do some tests of spray fixed samples because
the logistics of transporting liquids and specimens is getting more and
more difficult. Any comments would be greatly appreciated.


Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

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