Re: mounting media

From:Cathy Gorrie


I have recently started using a DAKO product called "Flourescent 
mounting medium" which is excellent. A bit on the expensive side but 
it is an aqueous mountant that dries relatively hard. At least the 
coverslips stay put and it doesn't dry back. I too got sick of the 
nail varnish saga.


Cathy Gorrie
Scientific Officer
Neural Injury Research Unit,
School of Anatomy,
University of New South Wales
Sydney, N.S.W. 2052

At 2:25 PM -0400 26/9/01, Martha Ward wrote:
>Martha Ward writes:
>I am looking for a permanent mounting media suitable for
>immunoflourescent staining on renal biopsies.  We are currently using
>Vectashield from Vector Laboratories and then we seal around the
>coverslip with clear nail polish.  We are looking for something that
>will not move around as much (dry a little harder) but still has
>quenching properties.  I saw a product mentioned in an article called
>gelvatol.  Has anyone tried it and could you tell me where to find it.
>Thanks in advance for your help.
>Martha Ward
>Wake Forest University Baptist Medical Center
>Molecular Diagnostics Lab
>Winston-Salem, NC

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