Hi Sanchez, I don't have a reference offhand, but endogenous biotin in
hepatocytes is a definite pitfall. Why not try a directly conjugated
anti mouse FITC ab with unlabelled HSA ab? This would eliminate the
biotin problem. In frozen sections lightly fixed, It should be
sensitive enough. I suppose you could use a biotin blocking procedure as
well. Also, another marker might be AFP. Cytokeratin 8/18 (Ie. CAM5.2)
is good for hepatocytes as well.
Hope this helps.
sanchez romeo wrote:
> Dear Histonetters,
> I am looking for a marker for hepatocytes in pig fetuses for use on
> frozen cryosections (immunoflourescence). I have previously used a
> commercial marker (hepatocyte specific antigen) with minimal success.
> In my recent stainings I noticed that streptavidin-FITC by itself
> appears to label only hepatocytes giving a distinct granular
> cytoplasmic stain. Is this an acceptable marker? Can anyone suggest a
> reference on endogenous biotin in fetal hepatocytes?
> Many thanks in advance.
> Romeo Sanchez (Philippines)
> PhD student
> University of Ghent, Belgium
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