Re: hepatocytes


Hi Sanchez,  I don't have a reference offhand, but endogenous biotin in 
hepatocytes is a definite pitfall.  Why not try a directly conjugated 
anti mouse FITC ab with unlabelled HSA ab?  This would eliminate the 
biotin problem.  In frozen sections lightly fixed, It should be 
sensitive enough. I suppose you could use a biotin blocking procedure as 
well.  Also, another marker might be AFP.  Cytokeratin 8/18 (Ie. CAM5.2) 
is good for hepatocytes as well.
Hope this helps.

sanchez romeo wrote:

> Dear Histonetters,
> I am looking for a marker for hepatocytes in pig fetuses for use on 
> frozen cryosections (immunoflourescence). I have previously used a 
> commercial marker (hepatocyte specific antigen) with minimal success. 
> In my recent stainings I noticed that streptavidin-FITC by itself 
> appears to label only hepatocytes giving a distinct granular 
> cytoplasmic stain. Is this an acceptable marker? Can anyone suggest a 
> reference on endogenous biotin in fetal hepatocytes?
> Many thanks in advance.
> Romeo Sanchez (Philippines)
> PhD student
> University of Ghent, Belgium
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