Re: epifluorescence visualization with pmma


pmma should not have an effect, but fixative can.  the fluorochromes are
water soluble, we use methanol because water based fixatives can dissolve
the label.
patsy ruegg

John Baker wrote:

> A forwarded question from one of our post docs.
> I am investigating vertebral growth rates in a murine scoliosis
> model.  I am wondering if PMMA embedding has an adverse effect on the
> vizualization of epifluorescence techniques, specifically calcien or
> xylenol?  Thanks always.
> Ryan
> --
> John A. Baker
> The University of Michigan
> Orthopaedic Research Laboratories
> Histology Unit
> 400 North Ingalls, G160
> Ann Arbor, MI 48109-0486
> Main lab office phone:734-763-9674
> Histology office:734-936-1635

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